Journal: Acta Pharmacologica Sinica

Article Title: Brain protection against ischemic stroke using choline as a new molecular bypass treatment

doi: 10.1038/aps.2015.104

Figure Lengend Snippet: Effects of oral choline treatment on angiogenesis in the ischemic brains. (A) Macroscopic images of the pial vasculature. (B) Microscopic images of ischemic cerebral cortex sections immunohistochemically stained using an anti-CD34 antibody to analyze vessel density, scale bar=20 μm. (C) Vessel density (n=6). (D) Number of microvessels in the cortex (n=3). Mean±SEM. bP<0.05, cP<0.01 vs sham; eP<0.05, fP<0.01 vs pMCAO.

Article Snippet: The capillary density was examined using a primary rabbit anti-rat CD34 antibody (Bioss, Beijing, China).

Techniques: Staining

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Involvement of stromal cell-derived factor-1α (SDF-1α), stem cell factor (SCF), fractalkine (FKN) and VEGF in TSG protection against intimal hyperplasia in rat balloon injury.

doi: 10.1016/j.biopha.2018.12.030

Figure Lengend Snippet: Fig. 5. TSG increased the protein expression of VEGF in serum and the protein expression of CD34 in vessel wall after balloon injury. A: The representative sections of arteries with immunostaining for CD34 (10 ×). B: The representative sections of arteries with immunostaining for CD34 (40 ×). C: Analysis for protein expression of VEGF in serum. n = 3-5. *P < 0.05 vs Sham group, #P < 0.05 vs Model group.

Article Snippet: CD34 goat anti-rat polyclonal antibody was purchased from R&D System (USA).

Techniques: Expressing, Immunostaining

Journal: Diagnostic and interventional radiology (Ankara, Turkey)

Article Title: Liver regeneration after portal vein embolization: comparison between absolute ethanol and N -butyl-cyanoacrylate in an in vivo rat model.

doi: 10.4274/dir.2022.211144

Figure Lengend Snippet: Figure 1. Summary of animal experiments. In total, 27 rats were included in this study. The rats received either sham treatment [5 (18.52%)] or PVE using absolute ethanol [5 (18.52%)] or NBCA [5 (18.52%)]. Then, 14 days after PVE, the liver weights were assessed. Six (22.22%) rats were used to analyze the necrotic area and IHC for CD68 and Ki-67 one day after PVE. The remaining six (22.22%) rats were used to evaluate portal endothelial injury 3 h after PVE via IHC for CD34. PVE, portal vein embolization; NBCA, N-butyl-2- cyanoacrylate; IHC, immunohistochemistry.

Article Snippet: The other contiguous sections were used for immunohistochemical staining for Ki-67 (diluted at 1:100; ab 16667; abcam plc, Cambridge, UK), CD68 (diluted at 1:100; MCA341GA; Serotec Co., Ltd., Sapporo, Japan), and CD34 (diluted at 1:100; AF4117; R&D Systems, Inc., Minneapolis, MN, US).

Techniques: Immunohistochemistry

Journal: Diagnostic and interventional radiology (Ankara, Turkey)

Article Title: Liver regeneration after portal vein embolization: comparison between absolute ethanol and N -butyl-cyanoacrylate in an in vivo rat model.

doi: 10.4274/dir.2022.211144

Figure Lengend Snippet: Figure 6. Immunohistochemistry for CD34 in the embolized lobe. (a, b) Embolized lobe after PVE using absolute ethanol (original magnification: 100×, 400×). (c, d) Embolized lobe after PVE using NBCA (original magnification: 100×, 400×). Injured endothelial cells embolized with absolute ethanol and NBCA after PVE are indicated (arrow). A thrombus is observed along the inner lumen of the portal vein (dagger). An NBCA cast adhesion is noted at the inner lumen of the portal vein (asterisk). PVE, portal vein embolization; NBCA, N-butyl-2-cyanoacrylate; P, portal vein; A, hepatic artery; B, bile duct.

Article Snippet: The other contiguous sections were used for immunohistochemical staining for Ki-67 (diluted at 1:100; ab 16667; abcam plc, Cambridge, UK), CD68 (diluted at 1:100; MCA341GA; Serotec Co., Ltd., Sapporo, Japan), and CD34 (diluted at 1:100; AF4117; R&D Systems, Inc., Minneapolis, MN, US).

Techniques: Immunohistochemistry

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Si-Miao-Yong-An on promoting the maturation of Vasa Vasorum and stabilizing atherosclerotic plaque in ApoE -/- mice： An experimental study.

doi: 10.1016/j.biopha.2019.108785

Figure Lengend Snippet: Fig. 3. Effect of Si-Miao-Yong-An (SMYA) on VV maturation in ApoE−/−mice. VV pericytes analysis by immunofluorescence double staining, endothelial cells were labeled with CD34, pericytes were labeled with Desmin. The results of immunofluorescence double staining showed that there was no plaque formation in aorta in the normal group. In the model group, a small pericytes collected new VV in aortic plaques. Compared with the model group, the density of mature VV in plaques in two treatment groups increased significantly (Magnification, ×400).

Article Snippet: Si-Miao-Yong-An (Lyophilized powder was composed of Scrophularia ningpoensis Hemsl., Lonicera japonica., Angelica Sinensis., Glycyrrhiza uralensis Fisch, preparing according to the ratio 3:3:2:1 and purchased from the first affiliated hospital of Tianjin university of Traditional Chinese Medicine); Simvastatin (Hangzhou MSD pharmaceutical co., Ltd., Hangzhou, China, specification was 20mg/tablets, Lot number: H20130146); L-methionine (M5308, sigma, USA); Hematoxylin-eosin staining kit (Tianjin baihao biotechnology co., Ltd.); Fluorescence antibody diluent (A1840, Beijing solaibao technology co., Ltd.); CD34 rat monoclonal antibody (GTX28158, GeneTex, USA); Desmin rabbit monoclonal antibody (ab32362, abcam); streptavidin–biotin complex with peroxidase (SABC-POD, Beijing zhongshan jinqiao biotechnology co., Ltd.) (rabbit IgG) ready-to-use kits and DAB Chromogenic Reagent Kit (Boster Biological Engineering Co., Ltd.); PrimeScriptTM RT reagent Kit (RR037A, Beijing baori medical technology co., Ltd.); SYBR®Premix Ex TaqTM II (RR420A, Beijing baori medical technology co., Ltd.).

Techniques: Double Staining, Labeling

Journal: Journal of Tissue Engineering and Regenerative Medicine

Article Title: In vivo analysis of vascularization and biocompatibility of electrospun polycaprolactone fibre mats in the rat femur chamber

doi: 10.1002/term.2868

Figure Lengend Snippet: HE staining, van Gieson staining, and immunohistochemical detection of CD 34: Representative histological stainings (a–o) 20 days after implantation into the femur chamber in Lewis rats. (a–c) HE staining of (1) granulation tissue, (2) implant, and (3) bone. (d–f) HE staining with higher magnification. (g–i) Collagen fibres were detected by van Gieson staining. (j–l) The presence of endothelial cells and accordingly the presence of vascular structures were confirmed by immunohistochemical detection of CD34. (m–o) Negative controls. Areas of the implant are marked (#), and arrows denote vascular structures [Colour figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: For detection of capillaries, endothelial cells were immunohistochemically stained using a rabbit anti‐rat CD34 antibody (Hoelzel Diagnostika Handels GmbH, Koeln, Germany).

Techniques: Staining, Immunohistochemical staining